– cell lysates, hypotonic solution lysis
– do Ub-VS experiment
– run gel, transfer, blot O/N
— run USP14 IP#7 lysates, transfer, blot alpha 7 O/N
– extract DNA from D1R-YFP, transform O/N
– autoclave any new media?
– dissociates EBs, and plate all of them (dissoc. well!!!)
– split two lines 9X 150mm.
– coat plates for cortical neurons 60mm x 8 and 24 well plate coverglass
– heart case!!! send out email
– email out DAC proposal.
– change to secondary for lab meeting
– develop after lab meeting
– pick colonies, do a start culture early in the morning be4 lab meeting, transfer to maxi culture at night.
– beg for neurons, plate neurons.
– maxi-prep. transfection if kit is around
– prepare for USP14 IP#8 – base on Western result, need to increase materials for IP???
– do a Ub-VS assay depending on result.
– add on agonist and do ICC? Talk to analysing core?
– ICC in the morning
– lunch with Pinar?
– Send Dan my DAC presentation
– do HBTM 235 homeworks, prepare for tutorial session.